glyXera

glyXera is specialist provider of high-performance glycoanalysis, with services and products customisable to meet specific client demands through cutting edge methods and tools. The company is an exclusive provider of glyXbox™, a high-performance glycoanalysis system.

With clients including some of the world’s leading pharmaceutical, biotechnology, and food companies, glyXera’s expertise guides clients toward improved decision-making, as well as boosting and increasing the speed of discovery phases, research and development (R&D), clinical trials, and eventual approval of products such as biopharmaceuticals vaccines and functional food.

The company’s stringent quality management solutions and operation protocols enable analyses to be replicated at the highest level, with transparency throughout.

Glycoanalytical solutions

glyXera has a proven track-record of providing glycoanalytical services. The company operates modern analytical and synthesis labs, which are equipped with state-of-the-art instrumentation.

glyXera provides high-performance glycoanalysis solutions for a worldwide client-base.

Glycoanalysis services can be customised to meet highly specific and unique demands.

Specialist scientific services provided include glycoprofiling and glycofingerprinting.

xCGE-LIF offers high capacities in throughput, reliability, sensitivity, and reproducibility.

High-performance glycoanalytical services are individualised according to specific client requirements through glyXera’s expertise and advanced technology platforms, with clients spanning academia, clinics, pharmaceutical, and food industries.

glyXera’s substantial experience covers glycomics, glycoproteomics and proteomics of biotechnological samples such as recombinant glycoproteins (such as monoclonal antibodies, fusion proteins, factors, and hormones) or viral membrane (glyco)proteins, as well as clinical samples such as blood, milk, and other body fluids.

A significant amount of care is put into R&D to develop, validate, implement, and apply cutting-edge glycoanalytical methods of a complementary or orthogonal nature, as well as dedicated kits, software tools, and databases.

Research is focused on developing and applying new high-performance glycoanalytical tools and techniques, in addition to specialist hardware and software, and customised reagents and kits.

High-performance glycoanalysis

glyXera’s glyXbox™ systems enable the generation and comparison of glycofingerprints from large sample sets and structural analysis of glycans, all based on highly reproducible retention, respectively, migration-times.

High-performance glycoanalysis is provided on three levels, comprising:

• Glycofingerprinting (GFP): glycosylation pattern analysis and comparison
• Standard Glycoprofiling (STD-GP): identification of glycans in complex mixtures via database matching
• Extended Glycoprofiling (EXT-GP): extended structural analysis of glycans in complex mixtures using exoglycosidase sequencing in combination with repeated glycoprofiling (including linkage analysis)

Exclusively, glyXera provides fast and reliable glycoanalysis by utilising a high-throughput system that offers optimum performance and superior capacities compared with similar tools available on the market. The company’s patented glyXbox^CE system is based on multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) provides:

• Up to 96 capillaries in parallel that enable ‘real’ high throughput (450x faster, than standard-HPLC systems)
• 3,500x higher sensitivity, than standard-HPLC systems
• 15x greater separation power than standard-HPLC systems
• High-reproducibility of peak heights (for quantitative analysis)
• Excellent reproducibility of migration times (for identification (qualitative analysis))

Glycoanalytical services

Glycoprofiling:
[from purified (glyco)proteins, (glyco)protein mixtures (separated by 1 or 2-dimensional gel electrophoresis) or from complex samples, such as: blood, plasma, serum, saliva, milk and tissue]

xCGE-LIF based:

• Glycofingerprinting (GFP)
• Standard Glycoprofiling (STD-GP)
• Extended Glycoprofiling (EXT-GP)

(xCGE-LIF = Multiplexed Capillary Gel Electrophoresis with Laser Induced Fluorescence Detection)

HILIC-FLR based:

• Glycofingerprinting (GFP)
• Standard Glycoprofiling (STD-GP)
• Extended Glycoprofiling (EXT-GP)

(HILIC-FLR = Hydrophilic Interaction Chromatography with Fluorescence Detection)

MALDI-Tof-MS(/MS) based glycoprofiling
(MALDI-Tof-MS(/MS) = Matrix Assisted Laser Desorption Time of Flight (Tandem) Mass Spectrometry)

PGC-LC-MS(/MS) based profiling of N- and/or O-glycans
(PGC-LC-MS(/MS) = Porous Graphitised Carbon Liquid Chromatography Electrospray Ionisation (Tandem) Mass Spectrometry)

Monosaccharide (Composition) Analysis:
Separation and quantification of enzymatically/chemically released:
Neutral monosaccharides (glucose, N-acetyl-glucosamine, galactose, N-acetyl-galactosamine, mannose, fucose)
Sialic acids (N-acetylneuraminic acid (NeuAc/NANA) and N-glycolylneuraminic acid (NeuGc/NGNA))
Terminal alpha galactose(s) (-Gal)

By: High pH Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), HILIC-FLR and/or Reversed Phase Liquid Chromatography with Fluorescence Detection (RP-LC-FLR)

Site Occupancy Analysis:
Determination of N- and O-glycosylation site occupancy of (monoclonal) antibodies and other (recombinant) (glyco)proteins by LC-MS/MS

Proteomics:
Protein identification via "Peptide Fragmentation Mass Fingerprinting", by MALDI-Tof-MS/MS and/or LC-ESI-MS/MS

N- & O-Glycopeptide Mapping:
Determination of glycan (composition) and glycosylation site by high resolution LC-ESI-MS

N- & O-Glycoproteomics:
Identification of glycopeptides and determination of the peptide and the glycan part

General (Glyco)protein Characterisation:
Analysis of size and purity of isolated (glyco)proteins or size distribution and composition of (glyco)protein mixtures via Protein Gel Electrophoresis Pattern Analysis by 1- or 2-dimensional gel electrophoresis

Glycoanalytical products

glyXbox^CE: High-performance glycoanalysis system based on Multiplexed Capillary Gelelectrophoresis with Laser Induced Fluorescence Detection (xCGE-LIF). Includes: SOPs for modular sample preparation and analysis and the glyXtool^CE software licence

glyXbox^LC: Standard glycoanalysis system based on Hydrophilic Interaction Chromatography with Fluorescence Detection (HILIC-FLR). Includes: SOPs for modular sample preparation and analysis and the glyXtool^LC software licence

Tailored reagents/kits for glycoanalysis

• Fluorescent tags
• Migration time normalisation standards
• Retention time normalisation standards
• Clean-up kits

About glyXera

German-based glyXera GmbH is a Max Planck Society spin-off, with offices and labs in Magdeburg, the state capital of Saxony-Anhalt. We are based in the ZENIT Building, at the Otto-von-Guericke University of Magdeburg’s medical campus.